Comprehensive Testing for Cigar Smoke and Filler

Enthalpy Specialty Labs offers a range of test methods for the collection and analysis of harmful and potentially harmful constituents (HPHCs) in cigar smoke and cigar tobacco filler.


Enthalpy Specialty Labs is a world leader in cigar testing using CORESTA cigar methods and in-house validated methods. Throughout the years, Enthalpy has tested thousands of varieties of cigars. Our testing methods allow us to test cigarillos, small cigars, large cigars, and premium cigars and offer the following:

  • Fully validated HPHC testing specific to cigars
  • The ability to test cigars of multiple lengths and ring gauge sizes
  • Advanced analytics on a per cigar level
  • Individual tracking for smoke analysis
  • Testing of up to 25 cigar brands per week

Enthalpy Specialty Labs has validated A2LA-Accredited Methods for the following HPHCs in cigars:

  • Carbonyl Compounds
  • Volatile Organic Compounds
  • Aromatic Amines
  • Polycyclic Aromatic Hydrocarbons (PAHs)
  • Metals
  • TNCO
  • Tobacco-Specific Nitrosamines (TSNAs)

Cigar Assays

Cigar Smoke
Method Description
AM-001 & AM-007 Nicotine and Water in Mainstream Smoke by GC (TNCO): Tar, Nicotine, Carbon monoxide, Water
AM-001 Menthol in Mainstream Smoke by GC
AM-011 Ammonia by HPLC
AM-015 Volatile Organics (MS) by GC/MS: 1,3-Butadiene, Isoprene, Acrylonitrile, Benzene, Toluene
AM-020 TSNAs-Nitrosamines in Mainstream Smoke: NAB, NAT, NNK, NNN
AM-044 Benzo[a]pyrene by GC/MS
AM-052 Metals by ICP/MS: Arsenic, Cadmium
AM-076 Selected Carbonyls in Mainstream Smoke by UHPLC with UV Detection: Formaldehyde, Acetaldehyde, Acrolein, Crotonaldehyde
AM-100 Selected Alkaloids in MS Smoke by GC/MS: Nornicotine, Anabasine
AM-180 VOCs I: Selected Volatiles in Mainstream Smoke by GC-MS: 1,3-Butadiene, Ethylene oxide, Isoprene, Propylene oxide, Vinyl acetate, Vinyl chloride
AM-193 VOCs II in Mainstream Smoke by GC-MS: Furan, Acrylonitrile, Nitromethane, Benzene, Toluene, 2-Nitropropane, Ethylbenzene, Styrene, 2,3-Benzofuran, Nitrobenzene, Naphthalene 
AM-199 Selected Primary Aromatic Amines (PAAs) in MS Smoke by GC-MS: 1-Aminonaphthalene (1-Naphthylamine), 2-Aminonaphthalene (2-Naphthylamine) , 4-Aminobiphenyl
AM-016 pH of Cigar Smoke
Cigar Tobacco Filler
Method Description
AM-031 TSNAs in Cigar Tobacco Filler: NAB, NAT, NNK, NNN
AM-072 Nicotine in Cigar Tobacco Filler by GC
AM-090 Humectants in Cigar Tobacco Filler by GC-FID: Glycerol, Propylene glycol, Triethylene glycol (TEG), Ethylene glycol (EG), Diethylene glycol (DEG)
AM-115 Ammonia in Cigar Tobacco Filler by HPLC
AM-180 VOCs I: Selected Volatiles in Cigar Tobacco Filler by GC-MS: 1,3-Butadiene, Ethylene oxide, Isoprene, Propylene oxide, Vinyl acetate, Vinyl chloride
AM-182 Nitrite in Tobacco by CFA
AM-218 Nitrate in Tobacco by Continuous Flow Analysis
AM-233 Water Activity
AM-053 pH of Cigar Tobacco Filler
TOX-011 Total Yeast/Mold Count – FDA BAM: 8ed. Ch18 Total Aerobic Plate Count – AOAC 966.23
Cigar Physical Parameters
Method Description
AM-009 Filter Length, Total Cigar Length, Tipping Paper / Overwrap Length, Circumference, Diameter, Tip Ventilation, Total Resistance to Draw (RTD or Pressure Drop) (VO/VC), Tobacco Filler Weight, Total Cigar Weight

Please reach out to our team for more information for your next validation project.